Research Projects

Analysis of PCL family of proteins during differentiation

CRG ­– Centre for Genomic Regulation

Barcelona

Spain

1/13

Supervision

Luciano Di Croce
CRG, 1st Supervisor

Giacomo Cavalli
CNRS, 2nd Supervisor

Objectives

Understand how the PCL family of proteins is implicated in modulating Polycomb binding and functions.

Methodology

Polycomb-group (PcG) proteins are key regulators of the transcriptional programs that maintain stem cell properties and dictate lineage specification. In mouse ESCs, PcG proteins encompass two enzymatically distinct complexes, the Polycomb Repressive Complex 1 (PRC1) and 2 (PRC2). Failure to properly control developmental transcription programs by Polycomb proteins is linked to disease and embryonic lethality. Genome-wide binding of PRC2 is modulated by ancillary proteins such as PCL family of proteins. Study the role of PCL1/2/3 in mouse ES cell and during differentiation into neurons and cardiomyocytes.

Expected Results

Revealing the role of PCL proteins in modulating occupancy of PRC2 complex in mouse ES cells and their role during differentiation.

Planned Secondments

CNRS, France (2 months):
Image analysis of undifferentiated and differentiated ES cells WT.
Modulation of the expression of PCL proteins and differentiation.
ELISAVA, Spain (2 weeks):
Design new ways to communicate science results.

Enrolment in doctoral programs

PhD in Biochemistry from Universitat Pompeu Fabra, Barcelona

References

Santanach, A. et al. The Polycomb group protein CBX6 is an essential regulator of embryonic stem cell identity. Nat Commun 8, 1235, doi:10.1038/s41467-017-01464-w (2017).

Ballare, C. et al. Phf19 links methylated Lys36 of histone H3 to regulation of Polycomb activity. Nat Struct Mol Biol 19, 1257-1265, doi:10.1038/nsmb.2434 (2012).